Noninvasive, Real-Time Method for the Examination of Thymidine Uptake Events—Application of the Method to V-79 Cell Synchrony Studies
✍ Scribed by Robert Graves; Rhian Davies; Gerard Brophy; Gerry O'Beirne; Neil Cook
- Publisher
- Elsevier Science
- Year
- 1997
- Tongue
- English
- Weight
- 212 KB
- Volume
- 248
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
✦ Synopsis
decay characteristics can be brought into proximity [ 14 C]Thymidine uptake into V-79 hamster lung fi-with the scintillant-containing base by virtue of the broblasts has been successfully demonstrated using a biological processes within the cells, thereby generatnoninvasive, real-time method utilizing Cytostar-T ing light. Figure 1 demonstrates the principle of the scintillating microplates. These plates are standard scintillating microplate. The signal generated is proformat, tissue culture-treated, 96-well microplates portional to the amount of radioisotope within the cell with an integral scintillating base. The microplates and can be detected and quantified using appropriate permit the culture and observation of adherent cell instrumentation.
monolayers. Biological activities of the cells can be