Noninvasive imaging of e-selectin expression by activated endothelium in urate crystal–induced arthritis

✍ Scribed by Peter T. Chapman; François Jamar; Andrew A. Harrison; Richard M. Binns; A. Michael Peters; Dorian O. Haskard

• Publisher: John Wiley and Sons
• Year: 1994
• Tongue: English
• Weight: 537 KB
• Volume: 37
• Category: Article
• ISSN: 0004-3591
• DOI: 10.1002/art.1780371207

✦ Synopsis

Objective. To assess the expression of the cytokineinducible endothelial leukocyte adhesion molecule Eselectin during the evolution of urate crystal-induced arthritis, using a recently described radiolabeled monoclonal antibody (MAb) imaging technique.

Methods. Monosodium urate (MSU) crystals and saline alone were injected respectively into the right (inflamed) and left (control) knees of 3 young pigs. Four hours later, "lIn-labeled 1.2B6 F(ab'), (anti-E-selectin MAb) and '251-labeled MOPC 21 F(ab'), (control MAb) were injected intravenously. Uptake of 1.2B6 in inflamed and control joints was assessed by scintigraphy 7 and 24 hours after intraarticular injection of MSU crystals. Immunohistochemistry studies and radioactivity counting of tissues were performed postmortem to confirm the observations from scintigraphy.

Results. MAb 1.2B6 F(ab')2 scintigraphic images of the knees revealed a significantly increased uptake in the right (inflamed) knee at 7 and 24 hours postinjection, particularly over the joint space. These in vivo images were consistent with E-selectin expression in the inflamed tissue detected by immunohistochemistry and with radioactivity counts postmortem. The synovial