Nonfluorescent negative stain for alanopine dehydrogenase activity on starch gels

✍ Scribed by G.P. Manchenko

• Book ID: 102629814
• Publisher: Elsevier Science
• Year: 1985
• Tongue: English
• Weight: 352 KB
• Volume: 145
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(85)90366-5

✦ Synopsis

A two-step procedure for negative staining NADHdependent alanopine dehydrogenase (ALPDH) activity on starch gels is described, using nitroblue tetrasolium and phenasine methosulfate. This method gives a blue background with unreacted NADH, and white areas where ALPDH is located. Using this technique the first reliable evidence of the existence of true ALPDH isoenzymes is obtained. o 1985 Academic press, hc.