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Nonenzymatic NADPH-dependent reduction of 2,6-dichlorophenol-indophenol

✍ Scribed by C. Dupuy; J. Kaniewski; R. Ohayon; D. Dème; A. Virion; J. Pommier

Book ID
102630466
Publisher
Elsevier Science
Year
1990
Tongue
English
Weight
442 KB
Volume
191
Category
Article
ISSN
0003-2697

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✦ Synopsis

The reduction of 2,6-dichloroindophenol (DCIP) by direct interaction with NADPH was studied. The results indicate that reduction proceeds via a direct electron transfer from NADPH to DCIP, with no oxygen consumption, and a rate constant of k = 4.69 M-1.s-1. The reduced DCIP can rapidly transfer its electrons to potassium ferricyanide (K3Fe(CN)6) or ferricytochrome c, but not to nitro blue tetrazolium. Superoxide dismutase inhibits DCIP reduction in an oxygen-dependent manner by favoring the reoxidation of the reduced DCIP. We therefore conclude DCIP is not suitable for detecting O2- when the nucleotides NADH or NADPH are present.

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Kinetic and mechanistic study of the reduction of 2,6-dichlorophenol indophenol by dithionites
✍ C.N Konidari; M.I. Karayannis 📂 Article 📅 1989 🏛 Elsevier Science 🌐 English ⚖ 666 KB

The reaction of 2,6-dichlorophenolindophenol (DCPI) and dithionites is studied kinetically by applying the stopped-flow technique. Reaction rate constants are given for the pH range 1.30-6.80. The reaction was found to follow first-order kinetics with respect to each of the reactants. For pH 3.97,5.