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Nonenzymatic chemiluminescent detection and quantitation of total protein on Western and slot blots allowing subsequent immunodetection and sequencing

โœ Scribed by F. Javier Alba; Dr. Joan-Ramon Daban


Publisher
John Wiley and Sons
Year
1997
Tongue
English
Weight
746 KB
Volume
18
Category
Article
ISSN
0173-0835

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The use of short peptide affinity tag sequences has become commonplace for the expression and purification of recombinant proteins. Many of these tags are antibody epitopes and detection of tagged proteins via Western blots is straightforward. However, the most common affinity tag used at present fo