A simple and effective procedure for the isolation and purification of gangliosides by preparative thin-layer chromatography is described. The method is based on nondestructive visualization of gangliosides on silica gel-precoated thin-layer chromatography plates by staining with uncharged lipophili
✦ LIBER ✦
Nondestructive Detection of Neutral Glycosphingolipids with Lipophilic Anionic Fluorochromes and Their Employment for Preparative High-Performance Thin-Layer Chromatography
✍ Scribed by Johannes Müthing; Sven E. Kemminer
- Publisher
- Elsevier Science
- Year
- 1996
- Tongue
- English
- Weight
- 217 KB
- Volume
- 238
- Category
- Article
- ISSN
- 0003-2697
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A new method for the detection of gangliosides based on the lipophilic fluorescence agent 4-(N,Ndihexadecyl)amino-7-nitrobenz-2-oxa-1,3-diazole (NBD dihexadecylamine) and its application for preparative high performance thin layer chromatography is described. Brain gangliosides were chromatographed