Nondestructive analyses of unsaturated fatty acid species in dietary oils by attenuated total reflectance with Fourier transform IR spectroscopy
✍ Scribed by Satoshi Yoshida; Hiroshi Yoshida
- Publisher
- Wiley (John Wiley & Sons)
- Year
- 2003
- Tongue
- English
- Weight
- 1014 KB
- Volume
- 70
- Category
- Article
- ISSN
- 0006-3525
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✦ Synopsis
Abstract
The aim of this study was to develop a nondestructive method to quantitate relative amounts of n‐3 and n‐6 polyunsaturated fatty acid (PUFA) species in vegetable oils and oil seeds using Fourier transform IR spectroscopy (FTIR). The alkene CH stretching vibrations of unsaturated fatty acids in oils showed IR absorption bands with various peak positions and intensities at around 3010 cm^−1^, depending on the extent of unsaturation and PUFA species. With the aid of partial least‐squares regression analysis, the FTIR measurement could practically predict the content of each PUFA species in the oil to be tested. A calculation method was also presented to directly find PUFA species in oils from the FTIR spectra. This technique was applied to dried soybean seeds to demonstrate a nonhomogenous distribution of saturated fatty acids and PUFAs, as well as glycans, in soybean cross sections. © 2003 Wiley Periodicals, Inc. Biopolymers (Biospectroscopy) 70: 604–613, 2003