Noncontact versus contact imaging: An atomic force microscopic study on hepatic endothelial cells in vitro
✍ Scribed by Filip Braet; Ronald De Zanger; Steffan Kämmer; Eddie Wisse
- Publisher
- John Wiley and Sons
- Year
- 1997
- Tongue
- English
- Weight
- 220 KB
- Volume
- 8
- Category
- Article
- ISSN
- 0899-9457
No coin nor oath required. For personal study only.
✦ Synopsis
Liver sinusoidal endothelial cells (LEC) contain fenesdiffer from endothelial cells in other capillaries by the presence trae, which control the exchange of fluids, solutes, and particles beof open fenestrae and the lack of a diaphragm and basal lamina. tween the sinusoidal blood and the microvillous surface of the paren-Fenestrae have a dimension on the order of 150-200 nm, which chymal cells. The surface of LEC can be imaged by scanning electron makes it necessary to use microscopes with a resolution better microscopy (SEM) and atomic force microscopy (AFM). SEM and than that obtained using the light microscope. One role of the AFM images of LEC can be used to study dynamic changes in fenesfenestrae is filtration or sieving of fluids entering the space of trae by comparing specimens fixed after different experimental treat-Disse, allowing only particles smaller than 150-200 nm to reach ments. In this article, we report the different results obtained when the parenchymal cells or to leave the space of Disse [1]. Endothecontact (using a constant force) or noncontact (amplitude detection) lial fenestrae are dynamic structures whose diameter and number imaging on the same cells was applied. Special attention was paid on the optimalization of the image acquisition of fenestrae, because vary in response to different hormonal, pharmacologic, and miquality SEM examinations of fenestrae have already extensively been crofilament-disrupting agents [2]. Current investigation revealed described. The following advances and conclusions are presented
that the state of organization of the actin cytoskeleton is important here: 1) High-resolution imaging of slightly fixed LEC in fluid can be in the dynamics of LEC fenestrae and that the actin cytoskeleton performed in noncontact AFM; 2) correct acquisition of images of of LEC is probably the main regulator for sieving between the fenestrae with regard to their size (M, {200 nm) and shape (oval, sinusoidal blood and the parenchymal cells .
without deformation) under liquid was possible with noncontact AFM, Since its invention by Binnig et al. [5], atomic force microswhich was hitherto only feasible with fixed, dried, and coated LEC in copy (AFM), also called scanning force microscopy, has been contact AFM or SEM; 3) this mode of operation is more gentle to cells than contact mode; 4) images of LEC obtained in noncontact