Non-uniform distribution of methylatable CCGG sequences on human chromosomes as shown by in situ methylation
✍ Scribed by Carlos Sentís; Paloma Ludeña; Jose Fernandez-Piqueras
- Publisher
- Springer
- Year
- 1993
- Tongue
- English
- Weight
- 983 KB
- Volume
- 102
- Category
- Article
- ISSN
- 0009-5915
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✦ Synopsis
We carried out in situ methylation of human chromosomes with the HpaII methylase using [3H]methyl-S-adenosyl-L-methionine as the methyl group carrier. Autoradiographs localising [3H]methyl groups show methylatable CCGG sequences in the Rbands as well as in the short arms of the acrocentric chromosomes that include ribosomal DNA. The strongest labelling was observed over a subset of Rbands, including T-bands. Since methylatable CCGG sequences are representative of the unmethylated fraction of DNA, we suggest that differences in the degree of DNA methylation could be involved in the structure and function of chromosomal bands.