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Non-steroidal anti-inflammatory drugs inhibit telomerase activity in head and neck squamous carcinoma cell lines

✍ Scribed by Dietmar Thurnher; Maia Bakroeva; Michael Formanek; Birgit Knerer; Johannes Kornfehl

Publisher
John Wiley and Sons
Year
2001
Tongue
English
Weight
166 KB
Volume
23
Category
Article
ISSN
1043-3074

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✦ Synopsis

Abstract

Background

Antiproliferative effects in neoplastic cells of different origin have been attributed to non‐steroidal anti‐inflammatory Drugs (NSAIDs) during the past few decades.

Methods

We tested the influence of NSAIDs and hydrocor‐tisone on cell lines derived from head and neck squamous cell cancer (HNSCC) and on normal oral mucosal keratinocytes. Cell numbers were assayed by cell counting, proliferation, telomerase activity with a colorimetric assay, and cell cycle distribution by flow cytometry.

Results

In the neoplastic cell lines indomethacin and ibuprofen caused a dose‐dependent reduction of cell numbers and telomerase activity without altering cell viability and increased the percentage of cells in G0/G1 phase. In normal oral mucosal keratinocytes, only minor effects could be detected in response to NSAIDs and hydrocortisone.

Conclusion

These results demonstrate that NSAIDs have activity against HNSCC cells in vitro and may have clinical applications in combination with other therapeutic regimens. © 2001 John Wiley & Sons, Inc. Head Neck 23: 1049–1055, 2001.

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