Non-specific tritiation of some carcinogenic aromatic amines

✍ Scribed by G. E. M. Breeman; F. M. Kaspersen; J. G. Westra

• Publisher: John Wiley and Sons
• Year: 1978
• Tongue: French
• Weight: 337 KB
• Volume: 14
• Category: Article
• ISSN: 0022-2135
• DOI: 10.1002/jlcr.2580140512

✦ Synopsis

2-Aminofluorene, k-amino-3-methylbipheny1, 4-aminobiphenyl and 4-amino-4'-fluorobiphenyl were tritiated by acid catalyzed exchange of the corresponding nitro compounds followed by catalytic reduction. The exchange reactions were carried out by heating the nitro compounds in [ 'HI-trifluoroacetic acid with a catalytic amount of trifluoromethanesulphonic acid (TFMS). No l o s s of tritium could be detected during the conversion of the tritiated nitro compounds into the corresponding amines by catalytic hydrogenation. Incorporation into the ortho position is very low (<4$). During the metabolic activation and binding of the tritiated Nacetyl-2-aminofluorene to rat liver DNA in vivo, no tritium exchange occurred.