Non-selective precultivation of bacteria able to degrade different polycyclic aromatic hydrocarbons (PAH)
✍ Scribed by B. Mahro; K. Rode; V. Kasche
- Publisher
- John Wiley and Sons
- Year
- 1995
- Tongue
- English
- Weight
- 613 KB
- Volume
- 15
- Category
- Article
- ISSN
- 0138-4988
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✦ Synopsis
The influence of the pmultivation with different carbon sources on the ability of three different bacterial strains (Sphingomonas sp. strain BA2, Gordona sp. strain BW, Mycobacterium sp. strain VFI) to grow on phenanthrene. anthracene. pyrene or fluoranthene as the sole source of carbon and energy were studied. The strains were found to maintain their ability to grow on two of the four PAH after 30 serial transfers in liquid nutrient broth medium without selective pressure. The ability to grow on these PAH as the sole carbon and energy source was also maintained after curing experiments with acridine orange. The high stability of the PAH-degradation phenotype suggests that the tested strains carry at least parts of the PAH-degradation pathway genes on the chromosome. The PAH-degradation versatility of the strains was also influenced by the carbon source being used for precultivation. Possible reasons for the particularly good impact of the precultivation on hexadecane on the PAH degradation are discussed in this paper.