Non-selective interactions of concanavalin A with phosphatidylcholine small unilamellar vesicles: implications for chemical sensor development
✍ Scribed by Joseph S. Tauskela; Michael Thompson
- Publisher
- Elsevier Science
- Year
- 1992
- Tongue
- English
- Weight
- 379 KB
- Volume
- 264
- Category
- Article
- ISSN
- 0003-2670
No coin nor oath required. For personal study only.
✦ Synopsis
Addltlon of Concanavahn A (Con A) and succmyl-Con A (sue-Con A) to suspensions of phosphatldylchohne small undamellar vesicles (WV), Internally loaded with the dye, 6-carboxyfluorescem, caused an Increase m fluorescence over several hours This mdlcates that the presence of protem-polysacchande aggregates was not reqmred to alter WV membrane permeatnhty behavlour, m direct contrast to fmdmgs previously observed for anchored planar bdayer hpld membranes It IS proposed that sue-Con A molecules penetrated the WV membrane, creatmg enough of a structural change m the bllayer matrix so as to alter permeablhty behavlour, but not affect membrane mtegnty These results suggest that development of chemical sensors employmg aggregation or any other interaction which perturbs the hpld matrur sufficiently to alter electrochenucal behavlour may be hindered due to Interferences resulting from non-speak adsorptlon of protems to pbosphohpld membranes