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Non-radioactive PCR method for quantification of HIV-infected peripheral blood mononuclear cells in HIV-positive subjects

โœ Scribed by M. van der Feltz; H. S. L. M. Nottet; M. R. Visser; J. C. C. Borleffs; J. Verhoef

Publisher
Springer
Year
1993
Tongue
English
Weight
436 KB
Volume
12
Category
Article
ISSN
0934-9723

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The clinical performance of a modified polymerase chain reaction (PCR) testing algorithm was evaluated for confirming the presence of HIV-1 proviral DNA in peripheral blood mononuclear cells. A whole cell lysate, rather than phenol-purified DNA, was used for PCR amplification, under systematically o