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Non-immunological detection of PhoA fusion proteins

✍ Scribed by Linda H. Phillips; LaShauna Chambers; Ricardo Belmares; David W. Niesel


Book ID
119126214
Publisher
Elsevier Science
Year
1992
Tongue
English
Weight
963 KB
Volume
16
Category
Article
ISSN
0167-7012

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We studied the efficiency of the pHly152-derived haemolysin transport system using PhoA-HlyA fusion proteins and different constructs which provide HlyB/HlyD in trans. The optimal C-terminal HlyA signal consists of the last 60 amino acids. Longer stretches of HlyA do not improve the transport effici