## Abstract Human monolayer cells (HEp‐2 and Hep G2) were cultured in 96‐well plates. A modified Triton X 100 nonionic detergent extraction method was used for releasing intracellular ATP and protein in one step. The detergent technique was compared to perchloric acid (PCA) extraction. ATP was dete
Non identity of non ionic detergent soluble (NIDS) protein and stratum corneum autoantigens
✍ Scribed by P. J. Dykes; D. L. Williams; R. Marks
- Publisher
- Springer-Verlag
- Year
- 1983
- Tongue
- English
- Weight
- 250 KB
- Volume
- 275
- Category
- Article
- ISSN
- 0340-3696
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✦ Synopsis
The relationship between naturally occurring circulating stratum corneum autoantibodies and experimentally produced antibodies to a non ionic detergent soluble (NIDS) protein fraction from human stratum corneum has been investigated. Using indirect immunofluorescence and indirect haemagglutination techniques, anti NIDS protein serum was shown not to inhibit the autoantibody-antigen reaction. Furthermore no direct interaction could be demonstrated between sheep red blood cells coated with stratum corneum autoantigen and anti NIDS protein serum. The NIDS protein antigens therefore represent a separate and distinct class of stratum corneum antigens.
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Normal and leukemic lymphocytes were subjected to lysis with a non-ionic detergent and 2.0 M NaCI. The intact nuclear DNA and the associated molecules resulting from these lyses (the nucleoids) were then separated from DNAdissociated molecules and analyzed by sodium dodecyl sulfate-polyauylamide gel