✦ LIBER ✦
Non-ideal tracer sedimentation equilibrium: a powerful tool for the characterization of macromolecular interactions in crowded solutions
✍ Scribed by Germán Rivas; Allen P. Minton
- Publisher
- John Wiley and Sons
- Year
- 2004
- Tongue
- English
- Weight
- 160 KB
- Volume
- 17
- Category
- Article
- ISSN
- 0952-3499
- DOI
- 10.1002/jmr.708
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✦ Synopsis
Abstract
Non‐ideal tracer sedimentation equilibrium is a technique devised to quantify the effect of high concentrations of unrelated macromolecules on the self‐ or hetero‐associations of dilute macromolecules. Principles and experimental techniques are reviewed, and previous experimental work summarized. A new analysis of experimental data is presented that requires no a priori assumptions regarding the nature of weak repulsive interactions between solute species and the concentrated (crowding) species. Published in 2004 by John Wiley & Sons, Ltd.