Non-H-2-associated genetic regulation of cytotoxic responses to hapten-modified syngeneic cells Effect on the magnitude of secondary response and helper T cell generation after in vivo priming
✍ Scribed by Hiromi Fujiwara; Gene M. Shearer
- Publisher
- John Wiley and Sons
- Year
- 1981
- Tongue
- English
- Weight
- 523 KB
- Volume
- 11
- Category
- Article
- ISSN
- 0014-2980
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✦ Synopsis
Abstract
The present study investigates the role of non‐H‐2 genes in controlling generation of the H‐2‐restricted, T cell‐mediated cytotoxic response against trinitrophenyl (TNP)‐modified syngeneic cells (TNP‐self). Spleen cells from C3H/He (H‐2^k^) or B10.BR (H‐2^k^) normal mice or from mice primed to TNP in vivo by skin painting with trinitro‐chlorobenzene were used (a) for in vitro sensitization to TNP‐self and (b) as a source of radioresistant helper cells for augmenting the TNP‐self cytotoxic T lymphocyte (CTL) response generated by normal syngeneic spleen cells. Although spleen cells from unprimed mice from these two strains exhibited a comparable CTL response in a primary culture, a strong difference was observed in a secondary CTL response after in vivo priming. CTL activities generated in the secondary culture were much stronger in C3H/He than in B10.BR strains. This difference in the magnitude of secondary CTL responses was paralleled by generation of strong and weak helper cell activity in C3H/He and B10.BR, respectively. No detectable difference was observed between the two H‐2^k^ strains in the lysability of target cells and ability of stimulating cells to activate the primed unirradiated cells and radioresistant helper cells. This genetic difference detected in the H‐2^k^ haplotype was also demonstrated in the H‐2^b^ haplotype, by using C3H.SW and C57BL/10 mice which bear non‐H‐2 background genes corresponding to C3H/He and B10.BR mice, respectively. These results indicate the existence of a control mechanism influenced by non‐H‐2 genes, in addition to the established H‐2‐linked gene control.