Non-everted oxygenated rat intestinal segments as a measure of neutral detergent fiber effects on iron absorption
✍ Scribed by Ken Lee; Jesus Salvador Garcia-Lopez
- Book ID
- 103973978
- Publisher
- Elsevier Science
- Year
- 1990
- Tongue
- English
- Weight
- 477 KB
- Volume
- 1
- Category
- Article
- ISSN
- 0955-2863
No coin nor oath required. For personal study only.
✦ Synopsis
Iron absorption in the presence of varying amounts and sizes of dietary fiber was measured. A method using non-everted rat intestinal segments perfused in oxygen was refined. Neutral detergent fiber (NDF), a component of dietary fiber, was extracted from cooked pinto bean (Phaseolus vulgaris). The NDF did not affect iron absorption in intestinal segments from iron replete rats. However, 4 and 6 mg of NDF/ml significantly decreased iron absorption in the intestinal segments from anemic rats. NDF with a smaller particle size of 0.125 mm increased iron absorption relative to that absorbed with 0.180 mm particles. Histological examination validated using non-everted intestinal segments perfused with oxygen as a method for studying dietary effects on iron absorption. Segments which are not everted are less prone to damage. Perfusion with oxygen maintained metabolic activity in the tissue during the experiment.