Non-destructive chromatographic indicator for (and quantitative analysis in) the paper chromatography of carbohydrate compounds convertible to barium salts
✍ Scribed by Wallace M. Pasika; Arthur C. West III
- Publisher
- Elsevier Science
- Year
- 1971
- Tongue
- English
- Weight
- 612 KB
- Volume
- 63
- Category
- Article
- ISSN
- 1873-3778
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✦ Synopsis
X0X-DESTRUCTIVE CIiRO3L+XTOGRAPHIC INDICATOR FOR @XD 'QLXSTIT~~TI~ AXALYSIS IN) THE PAPER CHROMATOGRAPHY OF CARBOHYDRATE COJIPOUXDS CONVERTIBLE TO B-4RIUM SALTS The _xope of aPPlication of Thoriu as a chromatographic indicator has been investigated for various barium salts and has been found useful in column, paper and thin-layer chromatography-A procedure based on the use of Thorin is described for quantitative amdysis in paper chromatography. A mnnnmmber of reprts t3 document the use of Thor-in [r(O-Arsonophenylazo)-z- nag&bob~,6-dkuPfonic acid dis&ium sak] in qualitative and quantitative analysis of inorgarnic ions With one exception2 where it is used in the direct titration of sulfate, Thorinn's general use 5s in soEation calorimetric determinations. Herein we report on the use of Thorin as a surface adsorption indicator (pink) for the title salts im spot tests, paper and column chromatography. The usefulness of Thorin Iies primrmarily in the fact that it does not chemically interact with the substrate wateriaI and that once this material has been located, the indicator can be removed from the substrate material by "suitable washing". This aspect is especially advantageous in small scale preparative cohunn chromatography. Another as~xxt that makes the indicator useful is in work with carbohydrate ionic species that do not contain functional groups (i.e., aldehydic, vicinal hydroxides, etc_) u~sary for the usual chemical chromatographic indicators. Provided the ionic species can be converted to the barium salt, one can use Thorin as the indicator. The scope of application of Thorin is indicated below-. Thorin was obtained from the Eastman Chemical Co. (stock no. 6745) and used as received. The spray sohtion A was prepared by dissolving 25 mg of Thorin in IOO ml of aqueous Fisher "gram-Pat" buffer, pH 4, obtained from the Fisher Scientific Co. The spray sohtion B contained 25 mg of Thor-in in xoo ml of distilled water. *To dmm ia~@&~ shotdcf be addressed, Chemistry Dcpartmctit.