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Non-cross-reactivity of antibodies to murine LDH-C4 with LDH-A4 and LDH-B4

✍ Scribed by Liang, Zhi-Guo ;Shelton, Joyce A. ;Goldberg, Erwin

Book ID: 102894794
Publisher: John Wiley and Sons
Year: 1986
Tongue: English
Weight: 561 KB
Volume: 240
Category: Article
ISSN: 0022-104X
DOI: 10.1002/jez.1402400312

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✦ Synopsis

The induction of infertility by immunization with the spermspecific lactate dehydrogenase, LDH-C4, suggests its use in a contraceptive vaccine. Development of an immunological contraceptive for human use, however, requires that there be no cross-reactions with somatic tissues. We have demonstrated, using enzyme-linked immunoabsorbence, solid-phase radioimmunoassay, and competitive inhibition radioimmunoassay, that antisera to LDH-CA is specific and does not cross-react with the somatic isozymes, LDH-A4 and LDH-B4.

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