Non-covalent enzyme-polyelectrolyte complexes as self-buffered catalysts for use in low-water organic media

✍ Scribed by Alexander V. Vakurov; Alexander K. Gladilin; Johann Partridge; Vadim V. Mozhaev; Andrey V. Levashov; Peter J. Halling

• Book ID: 104647668
• Publisher: Springer-Verlag
• Year: 1996
• Tongue: English
• Weight: 275 KB
• Volume: 10
• Category: Article
• ISSN: 0951-208X
• DOI: 10.1007/bf00157373

✦ Synopsis

When free chymotrypsin is used to catalyse hydrolysis of N-acetyl tyrosine ethyl ester in 90% acetonitrile, the reaction rate soon falls because of the accumulation of the acidic product. If the enzyme is used in the form of a suspended complex with polyacrylic acid, the polyelectrolyte acts as an acid-base buffer to permit extended reaction.