Non-Chromosomal Antibiotic Resistance in Bacteria, III: Isolation of the Discrete Transfer Unit of the R-Factor R1
β Scribed by Stanley N. Cohen and Christine A. Miller
- Book ID
- 123649585
- Publisher
- National Academy of Sciences
- Year
- 1970
- Tongue
- English
- Weight
- 783 KB
- Volume
- 67
- Category
- Article
- ISSN
- 0027-8424
- DOI
- 10.2307/59964
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The mini plasmids deriving from pKN102, a copy mutant of the antibiotic resistance factor R1drd-19 of E. coli, share a common DNA sequence of 2.6 kb, which carries the minimal functions for autonomous replication. By cloning of two PstI fragments of this region it could be demonstrated that the "bas
We describe here the isolation of a mutant derivative of the drug resistance factor R1 (Meynell and Datta, 1966) that carries a nonsense mutation in a gene determining resistance to penicillins. We have used this mutant R1 to isolate derivatives of Escherichia coli and Klebsiella pneumoniae that con