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Non-biological production of ethylene in the acetylene reduction assay for nitrogenase

✍ Scribed by Thake, Brenda ;Rawle, P. R.

Publisher: Springer-Verlag
Year: 1972
Weight: 256 KB
Volume: 85
Category: Article
ISSN: 0003-9276
DOI: 10.1007/bf00425142

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✦ Synopsis

Ethylene is formed as a result of contact between triehloroaeetie acid and the rubber serum cap liners commonly used in the acetylene reduction assay for nitrogenase activity. The amount of ethylene produced varies from one rubber liner to another, but the rate is approximately linear over an eight-day experimental period. Immediate withdrawal of a sample of the gas phase from the incubation bottle at the end of the experiment, and storage in another vessel is recommended as an alternative to the addition of any substance to terminate experiments.

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