✦ LIBER ✦

No involvement of Ki-ras or p53 gene mutations in colitis-associated rat colon tumors induced by 1-hydroxyanthraquinone and methylazoxymethanol acetate

✍ Scribed by Masumi Suzui; Naoki Yoshimi; Toshikazu Ushijima; Yoshinobu Hirose; Hiroki Makita; Aijin Wang; Tshihiko Kawamori; Takuji Tanaka; Hideki Mori; Minako Nagao

Publisher: John Wiley and Sons
Year: 1995
Tongue: English
Weight: 427 KB
Volume: 12
Category: Article
ISSN: 0899-1987
DOI: 10.1002/mc.2940120403

No coin nor oath required. For personal study only.

✦ Synopsis

I-Hydroxyanthraquinone (1-HA), which is present in some herbs, and methylazoxymethanol (MAM) acetate, a metabolite o f azoxymethane, show synergistic carcinogenicity in rat colon, and 1 -HA induces ulcerative changes with simultaneous severe inflammation o f the entire colon. In this study, mutations in Ki-ras (exons 1 and 2) and p53 (exons 4-7) were studied by polymerase chain reaction (PCR)-single-strand conformation polymorphism (SSCP) analysis. Of 18 adenomas and 38 adenocarcinomas induced in male F344 rats (52 tumors induced by 1-HA plus M A M acetate, three by 1-HA alone, and one by MAM acetate alone), no mutations in Kiras or p53 were detected under t w o conditions of PCR-SSCP analysis. Because human colon carcinomas from patients with ulcerative colitis have a very low incidence of Ki-ras mutation, this experimental system would be a good animal model o f human colon carcinomas with ulcerative colitis and of human colon carcinomas without Ki-ras or p53 mutations.

📜 SIMILAR VOLUMES

No involvement of APC gene mutations in

No involvement of APC gene mutations in ulcerative colitis–associated rat colon carcinogenesis induced by 1-hydroxyanthraquinone and methylazoxymethanol acetate

✍ Masumi Suzui; Toshikazu Ushijima; Naoki Yoshimi; Hitoshi Nakagama; Akira Hara; T 📂 Article 📅 1997 🏛 John Wiley and Sons 🌐 English ⚖ 94 KB 👁 2 views

A rat model for human ulcerative colitis (UC) has been developed by using 1-hydroxyanthraquinone (1-HA) to cause severe inflammation of colonic mucosa. 1-HA also has synergistic effects on the carcinogenicity of methylazoxymethanol (MAM) acetate in the rat colon. In this study, four adenomas and 16

Frequent mutations of the rat β-catenin

Frequent mutations of the rat β-catenin gene in colon cancers induced by methylazoxymethanol acetate plus 1-hydroxyanthraquinone

✍ Masumi Suzui; Toshikazu Ushijima; Roderick H. Dashwood; Naoki Yoshimi; Takashi S 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 172 KB 👁 1 views

Recent evidence suggests that the β-catenin gene (CTNNB1) acts as an oncogene, and some human colon tumors with an intact APC gene have activating mutations in CTNNB1. In this study, mutations in the region corresponding to N-terminal phosphorylation sites (codons 1-51) of the rat Ctnnb1 gene were i