No evidence for a tumor necrosis factor α stimulated 2-methylaminoisobutyric acid uptake in hepatocyte monolayer
✍ Scribed by Soo-Kyung Lim; Jean-Pascal de Bandt; Christian Aussel; Pascal Pernet; Jacqueline Giboudeau; Luc Cynober
- Publisher
- John Wiley and Sons
- Year
- 1995
- Tongue
- English
- Weight
- 580 KB
- Volume
- 162
- Category
- Article
- ISSN
- 0021-9541
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✦ Synopsis
This study investigates the short-tem effects of glucagon and human recombinant tumor necrosis factor a (TNFa) singly and in association on 2-methylaminoisobutyric acid (MeAIB) transport in hepatocyte monolayers. As expected, glucagon induced a time-dependent stimulation of MeAlB transport. In our experimental conditions, TNFa did not induce cytolysis. A 2 hour exposure to TNFa (0.05-500 ng/l) with or without glucagon (lo-" to l o p h M) did not modify the basal or glucagon-stimulated MeAlB transport. Varying the duration of exposure to TNFa 5 ngil up to 6 h was equally ineffective. The presence of hydrocortisone potentiated the glucagon-stimulated transport, but TNFa remained ineffective. Finally, the association of interferon (IFNy) with TNFa and/or glucagon was unable to modify the transport activity. These data demonstrate that TNFa does not exert a direct effect on MeAlB transport in hepatocytes, at least on a short-term basis.