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NMR titration curves of histidine ring protons. 11. Near-heme histidine residues of deoxy- and oxymyoglobins

โœ Scribed by Ohms, J. P.; Hagenmaier, H.; Hayes, M. B.; Cohen, J. S.


Book ID
127285305
Publisher
American Chemical Society
Year
1979
Tongue
English
Weight
475 KB
Volume
18
Category
Article
ISSN
0006-2960

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Fully deuterated yeast phosphoglycerate kinase ([2H]PGK) was prepared biosynthetically with only histidine side chains of normal (1H) isotopic composition. The 1H NMR spectrum of this enzyme ([1H]His[2H]PGK) showed that the histidine side chains are clearly visible as sharp signals. Thus detailed st