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NMR study of silk I structure of Bombyx mori silk fibroin with 15N- and 13C-NMR chemical shift contour plots

✍ Scribed by Tetsuo Asakura; Makoto Demura; Takeshi Date; Naoshi Miyashita; Katsuaki Ogawa; Michael P. Williamson

Publisher
Wiley (John Wiley & Sons)
Year
1997
Tongue
English
Weight
817 KB
Volume
41
Category
Article
ISSN
0006-3525

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✦ Synopsis

The me[u.stulrlt' .s[u[e silk 1.stractlires ofBombyx mori silk,iibroin in [he .sdid .stute tiw-e .s[udied on [he basix oj '>IV-and i 3C-nmr chemical sh(jis 03',4la, Ser. and Gl~residaes. The 1'N crmpolariza[imr magic angle spinning (CP/MAS) nmr spectra of the ptwipi[ated ji-ac[im ufier ch,twrc~(r> T).jin h! 'drol~'.sis gl"B. rnori silk Jibroin }vith the silk i und silk 11, /ijrm.s wt)re me a.) ured to de(ermitw [hc i 'N chemical ,sh(jis of Gly, .4la, and Ser rcsidmw. For comparison, 1'N CP/ .4!.4S nmr lhtvnical sh~jts (?j'.4la n,tve measw-edjbr ['> N] Ala Philosamia cyrithia ricini silk Iibroin wi[h arrtiparallel ~-shee[ and a-heli.~,fi)rms. The '-'C CPI.W,4S nmr chemical .shi[i.sqj" Ala, Ser. and GIJSresidues of B. mori silk. fibrwin with the silk I and silk 11.fin-tns, as wdl a.s '-{C CP/Al.4S nmr chemical shljis of ,41a residue of P. c. ncini silk ,fihroin ~i(h ~-sheet and a-heli.r jiwm.v, are u.wd,ii)r lhe ctaminatimr of [he silk 1.struc[ure. Bo[h silk I and ~-heli.~peaks are sh(fied (t) a lo~t'er.jie[dthun silk II (P-sheet) Stir the Ca carbons ?fthe.4 la rc~idue~, while holh C'Bcarbon peaks arc .sh(lied [o higher,tield. However, the silk I peak of the i 'N trtttleas oj'the Ala re.)ldue is .shilied[o Imter,field than [he silk 11peak, ba[ the a-hel[.v peak is .sh(lied to high jield Thu.$, the d(fliv-etr(e in the .struclure het weerr[he silk 1 und a-heli.y [.sre@ted in a dijjivetr[ tnanner het t{eetl [he 1(<'and )'N chemical shifis. The Cm and ('@them ical .sh![icon[our plo[ .Iin'.4Iu untl Ser rt>.~idlim, and the Ca PIOI .fi~r the Gly residw, i~ere prepared. frf)tn the Protein Data Bank da (u (hained,{ijr 1.?proteins and u.sed,n fi)rttla

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