NMR studies on self-complementary oligonucleotides conjugated with methylene blue

✍ Scribed by Judith Jähnchen; Maria G. M. Purwanto; Klaus Weisz

• Book ID: 101721170
• Publisher: Wiley (John Wiley & Sons)
• Year: 2005
• Tongue: English
• Weight: 427 KB
• Volume: 79
• Category: Article
• ISSN: 0006-3525
• DOI: 10.1002/bip.20363

✦ Synopsis

Abstract

A carboxyl‐functionalized methylene blue (MB) derivative was synthesized and covalently coupled to three CG‐rich self‐complementary 2′‐deoxyoligonucleotides at their 5′‐end. Thermodynamic and structural details about the interactions between the dye and oligonucleotide duplexes were investigated employing ultraviolet (UV) melting and ^1^H nuclear magnetic resonance (NMR) experiments. In contrast to previous findings on MB binding, no specific intercalation or binding in the minor or major groove of the double helix was found in a 100 m__M__ NaCl buffer. Rather, proton chemical shift changes in the conjugates provide ample evidence for weak dye–DNA interactions largely through external MB stacking on the terminal base pairs. © 2005 Wiley Periodicals, Inc. Biopolymers 79: 335–343, 2005

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