NMR studies of the 4Fe4S cluster in the hyperthermophilic, P. furiosus Ferredoxin
β Scribed by C.M. Gorst; G.N. LaMar; E. Smith; M.W.W. Adams
- Publisher
- Elsevier Science
- Year
- 1993
- Tongue
- English
- Weight
- 62 KB
- Volume
- 51
- Category
- Article
- ISSN
- 0162-0134
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The oxidized and reduced forms of the (Fe4-S4) ferredoxin I from Desulfovibrio desulfuricans Norway were investigated by 'H NMR spectroscopy with the aim of obtaining the complete assignment of the cysteines ligating the cluster. A combination of TOCSY and NOESY measurements together with informatio
The oxidized form of the ferredoxin I from Desulfovibrio desulfuricans Norway (DdN Fd I) [Fe 4 ΓS 4 ] was investigated by 1H and 13C NMR spectroscopy. The sequence-speciΓc 1H assignments of 93% of the amino acid residues of the whole protein, a complete determination of its secondary structure and