NMR studies of intracellular water at 300 MHz: T2-specific relaxation mechanisms in synchronized or EGF-stimulated cells
✍ Scribed by D. E. Callahan; S. F. Deamond; D. C. Creasey; T. L. Trapane; S. A. Bruce; P. O. P. Ts'O; L.-S. Kan
- Book ID
- 102952394
- Publisher
- John Wiley and Sons
- Year
- 1991
- Tongue
- English
- Weight
- 813 KB
- Volume
- 22
- Category
- Article
- ISSN
- 0740-3194
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✦ Synopsis
Abstract
Responses specific to the spin‐spin relaxation time (T~2~) have been observed in two time‐dependent studies of the intracellular water in normal and transformed Syrian hamster fetal fibroblasts. At 300‐MHz (7.0 T), the spin‐lattice relaxation time (T~1~) was insensitive to several aspects of cellular physiology that produced changes in the T~2~ and the apparent self‐diffusion coefficient (D~app~) of intracellular water. In normal cells stimulated with epidermal growth factor (EGF), T~1~ was insensitive to time‐dependent changes detected by T~2~ and D~app~. In synchronized tumor cells, T~1~ was insensitive to cell‐cycle‐dependent changes detected by T~2~. The strongly coupled behavior of T~2~ and D~app~ that was observed as a function of time in EGF‐stimulated cells indicates that the diffusion of intracellular water through inhomogeneous local magnetic field gradients produced effects observable in T~2~. Conformational changes in large intracellular macromolecular assemblies such as chromatin or the cytoskeleton may alter the magnitude and inhomogeneity of local field gradients, producing responses in T~2~ and D~app~ only. © 1991 Academic Press, Inc.