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NMR Spectroscopy of Paramagnetic Metalloproteins

✍ Scribed by Ivano Bertini; Claudio Luchinat; Giacomo Parigi; Roberta Pierattelli

Publisher
John Wiley and Sons
Year
2005
Tongue
English
Weight
433 KB
Volume
6
Category
Article
ISSN
1439-4227

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✦ Synopsis

Abstract

This article deals with the solution structure determination of paramagnetic metalloproteins by NMR spectroscopy. These proteins were believed not to be suitable for NMR investigations for structure determination until a decade ago, but eventually novel experiments and software protocols were developed, with the aim of making the approach suitable for the goal and as user‐friendly and safe as possible. In the article, we also give hints for the optimization of experiments with respect to each particular metal ion, with the aim of also providing a handy tool for nonspecialists. Finally, a section is dedicated to the significant progress made on ^13^C direct detection, which reduces the negative effects of paramagnetism and may constitute a new chapter in the whole field of NMR spectroscopy.

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