✦ LIBER ✦

NMR of peptides and proteins in oriented membranes

✍ Scribed by Marassi, Francesca M.

Publisher: John Wiley and Sons
Year: 2002
Tongue: English
Weight: 364 KB
Volume: 14
Category: Article
ISSN: 1043-7347
DOI: 10.1002/cmr.10022

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✦ Synopsis

Abstract

Solid‐state NMR spectroscopy is used to determine the structures of membrane peptides and proteins in lipid bilayers. The methodology for membrane protein structure determination using solid‐state NMR of oriented lipid bilayer samples is outlined. Recent developments in recombinant bacterial expression systems for the preparation of isotopically labeled membrane proteins, pulse sequences for high‐resolution spectroscopy, and structural indices that guide the structure assembly process greatly extend the capabilities of the technique. © 2002 Wiley Periodicals, Inc. Concepts in Magn Reson 14, 212–224, 2002.

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