NMR of Biological Macromolecules

✍ Scribed by Chariklia Ioannidou Stassinopoulou (auth.), Chariklia Ioannidou Stassinopoulou (eds.)

• Publisher: Springer-Verlag Berlin Heidelberg
• Year: 1994
• Tongue: English
• Leaves: 366
• Series: NATO ASI Series 87
• Edition: 1
• Category: Library

✦ Synopsis

Provided here are the latest techniques of NMR as applied to the study of proteins, carbohydrates and nucleic acids.
The first chapters are devoted to an introduction to NMR and parameters related to molecular structure and molecular interactions. NMR experiments from basic 1D to 2D, 3D and 4D, used in combination with isotopically labelled molecules, are described and a general strategy is presented for biomacromolecular structure determination. Subsequent chapters deal with more advanced principles and techniques and their applications to structural and dynamic processes involving biomacromolecules in solution. Advanced results on peptide, protein, oligosaccharide and nucleic acid structure and recognition are presented.

✦ Table of Contents

Front Matter....Pages I-VIII
Basic Pulsed NMR Experiments....Pages 1-15
Principles of Multidimensional NMR....Pages 17-36
Understanding Pulsed FTNMR Experiments....Pages 37-65
Computer-Assisted Structure Determination of Biomacromolecules by NMR....Pages 67-85
Nuclear Shielding and Biomolecular Structure....Pages 87-114
NMR Studies of Protein Ligand Interactions....Pages 115-154
NMR Studies of Ligand-Protein Interactions Involving Quadrupolar Nuclei....Pages 155-170
Multidimensional NMR Spectroscopy of Peptides....Pages 171-188
Methods used in the Assignments of the 1 H and 15 N resonances of the c-Abl src homology 2 (SH2) domain....Pages 189-198
NMR and Unpaired Electrons in Biomolecules....Pages 199-216
Long Time Protein Dynamics....Pages 217-232
Local Dynamics of Macromolecules....Pages 233-244
Structural and Conformational Studies of Glycoproteins and Oligosaccharide Recognition Determinants....Pages 245-262
13 C Nuclear Magnetic Relaxation of Carbohydrate Molecules in Solution....Pages 263-278
Dynamic Modelling of Polysaccharide Molecules in Solution....Pages 279-292
13 C- 1 H Spin-Coupling Constants in Furanosyl Rings: Application to Oligonucleotide Structure Determination....Pages 293-306
Through Bond Sugar-Phosphate Backbone Assignment in 13 C labeled RNA by Triple Resonance 1 H, 13 C, and 31 P NMR Spectroscopy....Pages 307-322
NMR Studies of Protein-Nucleic Acid Interaction: The LAC Operon....Pages 323-333
Determination of High-Resolution, Sequence-Dependent DNA Duplex Structures in Solution....Pages 335-348
Back Matter....Pages 349-364

✦ Subjects

Organic Chemistry;Cell Biology;Biophysics and Biological Physics;Condensed Matter Physics;Biochemistry, general