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NMR Microscopy of Single Neurons Using Spin Echo and Line Narrowed 2DFT Imaging

✍ Scribed by Richard W. Bowtell; Andrew Peters; Jonathan C. Sharp; Peter Mansfield; Edward W. Hsu; Nanci Aiken; Anthony Horsman; Stephen J. Blackband

Publisher
John Wiley and Sons
Year
1995
Tongue
English
Weight
687 KB
Volume
33
Category
Article
ISSN
0740-3194

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✦ Synopsis

Abstract

NMR microimages of single neural cells were acquired at 500 MHz using a conventional spin echo pulse sequence and a line‐narrowing sequence that eliminates susceptibility effects. The data show that any contribution to the measured T~2~ relaxation rate arising from diffusion in local field inhomogeneities using spin echo sequences at high fields and high spatial resolution is relatively small. We conclude that the measured T~2~ difference between the nucleus and cytoplasm in these cells represents primarily a true T~2~ relaxation effect arising from the interactions of water with macromolecules in the two compartments and does not result from microsusceptibility differences. These observations have implications regarding water compartmentation in single cells and the interpretation of the MR characteristics of tissues in vivo.

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