NMR Investigations of Fluorine-Labelled RNA: Application of Two-Dimensional Heteronuclear Experiments to Study Single-Strand Conformation
✍ Scribed by A. Kay Collier; John R. P. Arnold; Julie Fisher
- Publisher
- John Wiley and Sons
- Year
- 1996
- Tongue
- English
- Weight
- 427 KB
- Volume
- 34
- Category
- Article
- ISSN
- 0749-1581
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✦ Synopsis
The novel application of 2D heteronuclear NMR experiments to a single-stranded RNA fragment 5'-GCAU*GGCU*ACU*U*-3' labelled with 5-fluorouridine (U*) at four sites is reported. Heteronuclear equivalents of COSY and NOESY experiments are described, both implemented in the phase-sensitive mode. The phasesensitive heteronuclear COSY experiment allows the observation of the 19F-'H couplings, while comparison of heteronuclear NOESY spectra at various temperatures reveals exchange phenomena, not apparent in the magnitude mode or in one-dimensional experiments. These methods and observations could have important implications for the study of conformational flexibility in larger, functionally relevant RNAs.