NMR investigation of oligosaccharide conformation using dipolar couplings in an aqueous dilute liquid crystalline medium
✍ Scribed by Torgny Rundlöf; Clas Landersjö; Kristina Lycknert; Arnold Maliniak; Göran Widmalm
- Publisher
- John Wiley and Sons
- Year
- 1998
- Tongue
- English
- Weight
- 219 KB
- Volume
- 36
- Category
- Article
- ISSN
- 0749-1581
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✦ Synopsis
The tetrasaccharide lacto-N-neotetraose b-D-Galp-
Glcp was dissolved in a dilute mixture of dimyristoyl-and dihexanoylphosphatidylcholine in deuterium oxide and 1J(C,H) spinÈspin coupling constants were measured in an isotropic solution at 25 ¡C. On raising the temperature to 38 ¡C, an ordering of the system occurs which permits determination of dipolar couplings. An energy minimized molecular mechanics model was generated showing that the molecule is anisotropic and can be treated as a cylinder. A Ðt of the expression for the carbonÈproton dipolar couplings in a uniaxial molecule in a uniaxial liquid crystal revealed good agreement with experimental data, emphasizing the future potential of the method in studies of carbohydrate conformation.