## Abstract The reported competition STD NMR method combines saturation transfer difference (STD) NMR with competition binding experiments to allow the detection of high‐affinity ligands that undergo slow chemical exchange on the NMR time‐scale. With this technique, the presence of a competing high
✦ LIBER ✦
NMR-Based Screening of Membrane Protein Ligands
✍ Scribed by Naveena Yanamala; Arpana Dutta; Barbara Beck; Bart Van Fleet; Kelly Hay; Ahmad Yazbak; Rieko Ishima; Alexander Doemling; Judith Klein-Seetharaman
- Book ID
- 111368809
- Publisher
- John Wiley and Sons
- Year
- 2010
- Tongue
- English
- Weight
- 796 KB
- Volume
- 75
- Category
- Article
- ISSN
- 1747-0277
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## Abstract We demonstrate here a promising NMR method that provides evidence for chiral compound selector interaction as a first‐pass screening method. A novel adaptation of commonly used protein‐based screening technologies, this approach relies upon ligand‐to‐stationary phase interaction wherein