✍ Scribed by Torgny Rundlöf; Göran Widmalm
No coin nor oath required. For personal study only.
^1^H, ^13^C NMR coupling constants across the glycosidic linkages were measured for the trisaccharide α‐L‐Fuc__p__‐(1→2)‐β‐D‐Gal__p__‐(1→4)‐D‐Glc__p__ from human milk and its reduced analogue α‐L‐Fuc__p__‐(1→2)‐β‐D‐Gal__p__‐(1→4)‐D‐Glucitol employing one‐dimensional multiple‐site ^13^C excitation experiments. Hadamard spectroscopy and pulsed field gradients were also used with or without band‐selective ^1^H decoupling during the acquisition period. The experimental scalar coupling constants were compared to those calculated from Langevin dynamics simulations employing different molecular mechanics force fields. An analysis of ^3^J(C,H) values determined with this NMR technique on di‐ and trisaccharides is also performed. Copyright © 2001 John Wiley & Sons, Ltd.
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Proton-proton cross-relaxation rates have been measured for the trisaccharide beta-D-Glcp-(l --> 2)[beta-D-Glcp-(1 --> 3)]alpha-D-Glcp-OMe in D2O as well as in D2O/[D6]DMSO 7:3 solution at 30 degrees C by means of one-dimensional NMR pulsed field gradient 1H,1H NOESY and TROESY experiments. Interato