InTech, 2012. - 436 p. - ISBN: 9535107224 9789535107224
This book written by authors actively involved in the area of spin label/probe technique. The authors describe in detail some novel trends and analyze new approaches of practical applications of nitroxide radicals. The book will be of help to many scientists: chemists, physical chemists, biophysicists, biologists, physicians and other experts in a variety of disciplines, in which spin labels and probes are used, as well as to students and PhD students. It may be also suitable for teaching, and may help to promote the progress in natural sciences.
Contents
Theory
History of the Use of Nitroxides (Aminoxyl Radicals) in Biochemistry: Past, Present and Future of Spin Label and Probe Method
ESR Spectroscopy of Nitroxides: Kinetics and Dynamics of Exchange Reactions
Simulation of Rigid-Limit and Slow-Motion EPR Spectra for Extraction of Quantitative Dynamic and Orientational Information
Forty Years of the dl/d Parameter
Experiment
Spin Labels in the Gel Phase and Frozen Lipid Bilayers: Do They Truly Manifest a Polarity Gradient?
Magnetic and Electric Properties of Organic Nitroxide Radical Liquid Crystals and Ionic Liquids
pH-Sensitive Nitroxide Radicals for Studying Inorganic and Organo-Inorganic Materials and Systems
Synthesis and Utilization of Iยฑ-Substituted Nitroxides
Kinetics and Mechanism of Reactions of Aliphatic Stable Nitroxide Radicals in Chemical and Biological Chain Processes
Uniform EPR Spectra Analysis of Spin-Labeled Macro molecules by Temperature and Viscosity Dependences
Applications
In vivo Spectroscopy and Imaging of Nitroxide Probes
Fluorescent Nitrones for the Study of ROS Formation with Subcellular Resolution
Quantitative Determination of Thiol Status of Proteins and Cells by Nitroxyl Biradical .RS-SR.
Platinum Complexes with Bioactive Nitroxyl Radicals: Synthesis and Antitumor Properties
Use of Spin Trap Technique for Kinetic Investigation of Elementary Steps of RAFT-Polymerization