✦ LIBER ✦

Nitroxide TEMPOL impairs mitochondrial function and induces apoptosis in HL60 cells

✍ Scribed by Elena Monti; Rosanna Supino; Mariapia Colleoni; Barbara Costa; Raffaella Ravizza; Marzia B. Gariboldi

Publisher: John Wiley and Sons
Year: 2001
Tongue: English
Weight: 156 KB
Volume: 82
Category: Article
ISSN: 0730-2312
DOI: 10.1002/jcb.1160

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

The piperidine nitroxide TEMPOL induces apoptosis in a number of tumor cell lines through free radical‐dependent mechanisms. As mitochondria play a major role in apoptosis as both source and target for free radicals, the present study focuses on mitochondrial effects of TEMPOL in a human promyelocytic leukemic cell line (HL‐60). On 24‐h exposure to TEMPOL, the following alterations were observed: 1) decrease in both the intracellular and mitochondrial glutathione pools; 2) impairment of oxidative phosphorylation; and 3) decrease in mitochondrial membrane potential. In addition, TEMPOL was found to specifically target complex I of the respiratory chain, with minor effects on complexes II and IV, suggesting that mitochondrial effects might play a role in TEMPOL‐induced oxidative stress and apoptosis, and that TEMPOL might sensitize tumor cells to the pro‐apoptotic effects of cytotoxic agents. J. Cell. Biochem. 82: 271–276, 2001. © 2001 Wiley‐Liss, Inc.

📜 SIMILAR VOLUMES

Cycloprodigiosin hydrochloride, H+/CL– s

Cycloprodigiosin hydrochloride, H+/CL– symporter, induces apoptosis and differentiation in HL-60 cells

✍ Daigo Yamamoto; Yoshiko Uemura; Kanji Tanaka; Koji Nakai; Chizuko Yamamoto; Hiro 📂 Article 📅 2000 🏛 John Wiley and Sons 🌐 French ⚖ 215 KB 👁 1 views

Alkylphosphocholines induce apoptosis in

Alkylphosphocholines induce apoptosis in HL-60 and U-937 leukemic cells

✍ Spiro M. Konstantinov; Hansjörg Eibl; Martin R. Berger 📂 Article 📅 1997 🏛 Springer 🌐 English ⚖ 603 KB

Thymidine-dependent attenuation of the m

Thymidine-dependent attenuation of the mitochondrial apoptotic pathway in adenosine-induced apoptosis of HL-60 cells

✍ Chang-Mo Kang; Yousin Suh; Ik-Soon Jang; Sang Park 📂 Article 📅 2001 🏛 Springer-Verlag 🌐 English ⚖ 180 KB

Thymoquinone induces apoptosis through a

Thymoquinone induces apoptosis through activation of caspase-8 and mitochondrial events in p53-null myeloblastic leukemia HL-60 cells

✍ Mohamed A. El-Mahdy; Qianzheng Zhu; Qi-En Wang; Gulzar Wani; Altaf A. Wani 📂 Article 📅 2005 🏛 John Wiley and Sons 🌐 French ⚖ 597 KB

## Abstract Thymoquinone (TQ), the major biologically active component isolated from a traditional medicinal herb, __Nigella sativa__ Linn, is a potential chemopreventive and chemotherapeutic compound. Despite the promising antineoplastic activities of TQ, the molecular mechanism of its pharmacolog

Mitochondrial heterogeneity during staur

Mitochondrial heterogeneity during staurosporine-induced apoptosis in HL60 cells: Analysis at the single cell and single organelle level

✍ Stefano Salvioli; Jurek Dobrucki; Laura Moretti; Leonarda Troiano; Maria Garcia 📂 Article 📅 2000 🏛 John Wiley and Sons 🌐 English ⚖ 887 KB

Caspase inhibition and N6-benzyladenosin

Caspase inhibition and N6-benzyladenosine-induced apoptosis in HL-60 cells

✍ P. Mlejnek 📂 Article 📅 2001 🏛 John Wiley and Sons 🌐 English ⚖ 300 KB

## Abstract As an extension of our recently published work (Mlejnek and Kuglík [2000] J. Cell. Biochem. 77:6–17), the role of caspases in N^6^‐benzylaminopurine riboside (BAPR)‐induced apotosis in HL‐60 cells was evaluated in this study. Here, BAPR‐induced apoptosis was accompanied by activation of