Nitroxide spin labeled analogs of the non-ionic detergent triton X-100
โ Scribed by Richard B. Roman; John F.W. Keana
- Publisher
- Elsevier Science
- Year
- 1982
- Tongue
- English
- Weight
- 389 KB
- Volume
- 31
- Category
- Article
- ISSN
- 0009-3084
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โฆ Synopsis
Two nitroxide spin labeled analogs of the non-ionic detergent, Triton X-100, have been synthesized. Electron spin resonance-spectra of these compounds in solution, in egg lecithin multilayers, and in aqueous dispersion of dimyristoylphosphatidylcholine vesicles are described.
๐ SIMILAR VOLUMES
## Abstract Human monolayer cells (HEpโ2 and Hep G2) were cultured in 96โwell plates. A modified Triton X 100 nonionic detergent extraction method was used for releasing intracellular ATP and protein in one step. The detergent technique was compared to perchloric acid (PCA) extraction. ATP was dete
The release of sodium and potassium and the uptake of sucrose molecules was studied in pig lenses incubated in isosmotic sucrose solution in either the presence or absence of 1 % Triton X-100 (a non-ionic detergent). This Triton X-100 treatment has been shown to cause severe disruptions of cell memb