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Nitrogenase activity in the blue-green alga Plectonema boryanum strain 594

โœ Scribed by Stewart, W. D. P. ;Lex, M.


Publisher
Springer-Verlag
Year
1970
Weight
632 KB
Volume
73
Category
Article
ISSN
0003-9276

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โœฆ Synopsis


The non-heteroeystous filamentous blue-green alga, Plectonema boryanum strain 594 reduces acetylene to ethylene, incorporates 15N 2 into cell protoplasm, and grows readily in medium free of combined nitrogen, when incubated in a gas phase without added oxygen. Cells grown in the presence of 50 mg/1 of ammonium-nitrogen do not reduce acetylene, and a concentration of 0.015 atm. CO in the gas phase inhibits nitrogenase activity completely but inhibits 14C02 incorporation by only 28o/0 . Nitrogenase activity is inhibited after 2 h treatment with 3 โ€ข 10 -5 M DCMU and is inhibited completely in air.


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