Nitrogen Metabolism in Plants: Methods and Protocols

✍ Scribed by Kapuganti Jagadis Gupta

• Publisher: Springer New York;Humana
• Year: 2020
• Tongue: English
• Leaves: 182
• Series: Methods in Molecular Biology 2057
• Edition: 1st ed. 2020
• Category: Library

✦ Synopsis

This volume explores several different aspects of nitrogen metabolism, ranging from nitrogen uptake to assimilation. The chapters in this book cover topics such as measurement of activities of enzymes involved in nitrogen metabolism (i.e., nitrate reductase); measurement of nitric oxide, nitrite and ways to detect the N content in plants and microbes; characterization of root nodule bacteria (RNB); and techniques to perform proteomics of Extracellular Matrix (ECM). Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Thorough and cutting-edge, Nitrogen Metabolism in Plants: Methods and Protocols is a valuable tool for novice and expert researchers who are interested in learning more about this evolving field.

✦ Table of Contents

Front Matter ....Pages i-x
An Overview of Important Enzymes Involved in Nitrogen Assimilation of Plants (Reddy Kishorekumar, Mallesham Bulle, Aakanksha Wany, Kapuganti Jagadis Gupta)....Pages 1-13
Methods for Measuring Nitrate Reductase, Nitrite Levels, and Nitric Oxide from Plant Tissues (Aakanksha Wany, Pradeep Kumar Pathak, Kapuganti Jagadis Gupta)....Pages 15-26
Measurement of Nitrate Reductase Activity in Tomato (Solanum lycopersicum L.) Leaves Under Different Conditions (Mallesham Bulle, Reddy Kishorekumar, Pradeep K. Pathak, Aakanksha Wany, Kapuganti Jagadis Gupta)....Pages 27-35
Fluorimetric-Based Method to Detect and Quantify Total S-Nitrosothiols (SNOs) in Plant Samples (Paulo T. Mioto, Alejandra Matiz, Luciano Freschi, Francisco J. Corpas)....Pages 37-43
Measurement of S-Nitrosoglutathione Reductase Activity in Plants (Martina Janků, Tereza Tichá, Lenka Luhová, Marek Petřivalský)....Pages 45-59
Expression Analysis of Important Genes Involved in Nitrogen Metabolism Under Hypoxia (Mallesham Bulle, Reddy Kishorekumar, Aakanksha Wany, Kapuganti Jagadis Gupta)....Pages 61-69
Enzymatic Conversions of Glutamate and γ-Aminobutyric Acid as Indicators of Plant Stress Response (Alexander T. Eprintsev, Natalia V. Selivanova, Abir U. Igamberdiev)....Pages 71-78
Using Different Forms of Nitrogen to Study Hypersensitive Response Elicited by Avirulent Pseudomonas syringae (Namrata Singh, Pooja Singh, Pradeep Kumar Pathak, Kapuganti Jagadis Gupta)....Pages 79-92
Using Foldscope to Monitor Superoxide Production and Cell Death During Pathogen Infection in Arabidopsis Under Different Nitrogen Regimes (Reena Arora, Pooja Singh, Aprajita Kumari, Pradeep Kumar Pathak, Kapuganti Jagadis Gupta)....Pages 93-102
Methods for Estimation of Nitrogen Components in Plants and Microorganisms (Pratiksha Singh, Rajesh Kumar Singh, Qi-Qi Song, Hai-Bi Li, Li-Tao Yang, Yang-Rui Li)....Pages 103-112
A Precise Method for Analyzing Nitrogen Use in Foxtail Millet (Tirthankar Bandyopadhyay, Manoj Prasad)....Pages 113-118
Methods for Isolation and Characterization of Nitrogen-Fixing Legume-Nodulating Bacteria (Nisha Tak, Garima Bissa, Hukam S. Gehlot)....Pages 119-143
Detection and Quantification of Nitrifying Bacteria Using Real-Time PCR (Engil I. P. Pereira, Marcelo C. M. Teixeira Filho)....Pages 145-153
Extracellular Matrix Proteome: Isolation of ECM Proteins for Proteomics Studies (Eman Elagamey, Kanika Narula, Niranjan Chakraborty, Subhra Chakraborty)....Pages 155-172
Back Matter ....Pages 173-178

✦ Subjects

Life Sciences; Plant Sciences