Nitrogen metabolism during synchronous growth ofChlorella pyrenoidosa. I. Protein amino acid distribution

✍ Scribed by Hare, T. A. ;Schmidt, R. R.

• Publisher: Wiley (John Wiley & Sons)
• Year: 1965
• Tongue: English
• Weight: 357 KB
• Volume: 65
• Category: Article
• ISSN: 0095-9898
• DOI: 10.1002/jcp.1030650109

✦ Synopsis

Total cellular-N, acid soluble-N, lipid plus chlorophyll-N, and the levels of the individual amino acids were measured during synchronous growth of a high temperature strain, 7-11-05, of Clzlorella pyrenoidosa. Total cellular-N increased exponentially except for a small but reproducible deviation from log-linearity which occurred at approximately the middle of the 14 hour synchronous growth cycle. Although the level of acid soluble-N (as % of total cellular-N) exhibited definite perioclism during synchronous growth, the amount of nitrogen in this pool was small (approximately 4-8% ). Lipid-plus chlorophyll-N showed only slight fluctuations during cellular development; however, the trend followed that previously observed for phospholipid-P. The levels of the protein amino acids were expressed on a molepercentage basis of the total protein amino acids. The level of most of the protein amino acids remained essentially constant during the course of synchronous growth. The levels of the basic and acidic amino acids as well as alanine exhibited more significant periodism than the other protein amino acids. The total protein amino acid-N level comprised approximately 63 % of the total cellular-N throughout cellular development.