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Nitrogen fixation by the hydrogen-oxidizing bacteriumAlcaligenes latus

✍ Scribed by K. A. Malik; C. Jung; D. Claus; H. G. Schlegel

Publisher
Springer
Year
1981
Tongue
English
Weight
285 KB
Volume
129
Category
Article
ISSN
0302-8933

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✦ Synopsis

The aerobic hydrogen-oxidizing bacterium Alcaligenes latus represented by three strains was found to be able to grow with dinitrogen as the sole nitrogen source: The doubling time of total (Kjeldahl) nitrogen during growth on glucose at 30~ under an atmosphere containing 2 % (v/v) oxygen in dinitrogen amounted to 39h, while that in the presence of ammonium was 3h. Nitrogen fixation did apparently not occur under air. During diazotrophic growth the cells accumulated up to 75 % (w/dry weight) poly-/~hydroxybutyric acid. The efficiency of nitrogen fixation varied between 10 and 15 mg N per g glucose utilized. The specific nitrogenase activity measured in the acetylene reduction assay amounted to 5 -17 nmol CzH 4 formed per rain and mg protein.

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