Nitrite reduction in leaves; Studies on isolated chloroplasts
β Scribed by B. J. Miflin
- Book ID
- 104751859
- Publisher
- Springer-Verlag
- Year
- 1974
- Tongue
- English
- Weight
- 558 KB
- Volume
- 116
- Category
- Article
- ISSN
- 0032-0935
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β¦ Synopsis
Chloroplast preparations from spinach leaves containing a high percentage of intact chloroplasts were capable of light dependent nitrite reduction at rates around 9 ~mol/mg chlorophyll/h for at least 50 min. This reduction was inhibited by DCMU but unaffected by uncouplers of photosynthetic phosphorylation. Nitrite reduction was not accompanied by a stoichiometric evolution of oxygen evolution. The disappearance of nitrite was accompanied by an approximately stoichiometric formation of reduced nitrogen.
π SIMILAR VOLUMES
Isolated, intact spinach chloroplasts incubated in light in the presence of HC0 a-and NO~-have the greater proportion of their NADP present in the reduced form. The steady state concentration of NADPtt in light in these chloroplasts is significantly higher in tile presence of NO 2 than in its absenc
Nitrite reductase from barley seedlings was purified 50-60 fold by ammonium sulphate precipitation and gel filtration. No differences were established in the characteristics of nitrite reductases isolated in this way from either leaf or root tissues. The root enzyme accepted electrons from reduced m