Nitric oxide-releasing S-nitrosothiol-modified xerogels

✍ Scribed by Daniel A. Riccio; Kevin P. Dobmeier; Evan M. Hetrick; Benjamin J. Privett; Heather S. Paul; Mark H. Schoenfisch

• Book ID: 104003864
• Publisher: Elsevier Science
• Year: 2009
• Tongue: English
• Weight: 722 KB
• Volume: 30
• Category: Article
• ISSN: 0142-9612
• DOI: 10.1016/j.biomaterials.2009.05.006

✦ Synopsis

The synthesis, material characterization, and in vitro biocompatibility of S-nitrosothiol (RSNO)-modified xerogels are described. Thiol-functionalized xerogel films were formed by hydrolysis and co-condensation of 3-mercaptopropyltrimethoxysilane (MPTMS) and methyltrimethoxysilane (MTMOS) sol-gel precursors at varying concentrations. Subsequent thiol nitrosation via acidified nitrite produced RSNOmodified xerogels capable of generating nitric oxide (NO) for up to 2 weeks under physiological conditions. Xerogels also exhibited NO generation upon irradiation with broad-spectrum light or exposure to copper, with NO fluxes proportional to wattage and concentration, respectively. Xerogels were capable of storing up to w1.31 mmol NO mg À1 , and displayed negligible fragmentation over a 2week period. Platelet and bacterial adhesion to nitrosated films was reduced compared to non-nitrosated controls, confirming the antithrombotic and antibacterial properties of the NO-releasing materials. Fibroblast cell viability was maintained on the xerogel surfaces illustrating the promise of RSNOmodified xerogels as biomedical device coatings.