Nitric Oxide Protocols Volume 279 || Measurement of Nitric Oxide Synthase Activity In Vivo and In Vitro by Gas Chromatography–Mass Spectrometry
✍ Scribed by Hassid, Aviv
- Book ID
- 121329373
- Publisher
- Humana Press
- Year
- 2004
- Weight
- 224 KB
- Category
- Article
- ISBN-13
- 9781592598076
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📜 SIMILAR VOLUMES
Nitric oxide (NO) is formed by a class of NO synthases (NOS), which convert arginine into citrulline. A decreased in vivo NO availability can be the result of an increased NO inactivation or a decreased NO production. The latter can be assessed by measurement of isotopic enrichment of plasma citrull
A simple and sensitive assay method for NO synthase activity is described. Using glassy carbon as electrode and 30% methanol solution with 10mM NH&I as mobile phase, NO, can be measured without disturbing ECD-detectable substance in NO synthase assay mixture. The NO; production in the assay mixture