Nisoldipine blocks the increase of intracellular free calcium-ion concentration associated with elevated sodium-lithium countertransport activity in erythrocytes in patients with NIDDM
✍ Scribed by Fujita, J.; Tsuda, K.; Obayashi, H.; Fukui, I.; Ishida, H.; Seino, Y.
- Publisher
- John Wiley and Sons
- Year
- 1997
- Tongue
- English
- Weight
- 122 KB
- Volume
- 14
- Category
- Article
- ISSN
- 0742-3071
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✦ Synopsis
To understand the mechanism by which elevated sodium-lithium countertransport activity (SLC) associates with increased intracellular free calcium-ion concentration ([Ca 2+ ] i ), we investigated the relationship between SLC and the effects of the extracellular Ca 2+ concentration ([Ca 2+ ] o ) and a Ca 2+ -channel blocker, nisoldipine, on [Ca 2+ ] i in erythrocytes from 48 patients with non-insulin-dependent (Type 2) diabetes mellitus (NIDDM). There was a significant correlation between SLC and [Ca 2+ ] i . Nisoldipine in the incubation medium significantly decreased [Ca 2+ ] i , and there was a significant positive correlation between SLC and the degree of [Ca 2+ ] i decrease. When the [Ca 2+ ] o was elevated, [Ca 2+ ] i was significantly increased, but nisoldipine almost completely suppressed this increase of [Ca 2+ ] i . There was a significant positive correlation between SLC and the degree of the suppression. These data suggest that elevated SLC correlates with increased [Ca 2+ ] i , and that the increased [Ca 2+ ] i might be due to the increased Ca 2+ influx through a dihydropyridine-sensitive Ca 2+ pathway.